![sarawak borneo sarawak borneo](http://www.guideoftheworld.com/wp-content/uploads/map/kuching_sarawak_malaysia.jpg)
The PCR products were purified by precipitation with polyethylene glycol (10% PEG 6000 and 1.25 M NaCl in the precipitation mixture) and sequenced in both directions by Sanger method at Macrogene Europe. All PCRs were performed with Kapa polymerase (Kapa Biosystems) following a standard protocol with 37 to 40 cycles and annealing temperature of 56☌ (rDNA, atpA) or 47 ☌ ( matR). The mitochondrial genes atpA and matR were amplified and sequenced with primers developed by Eyre-Walker & Gaut ( 1997) and primers 26F and 1002R (Meng & al. 1990) and large subunit of ribosomal DNA (LSU rDNA) by primers N-nc26Srev ( Kuzoff & al. The small subunit of ribosomal DNA (SSU rDNA) was amplified and sequenced with primers NS1 and NS6, internal transcribed spacers of ribosomal DNA (ITS) with primers ITS1 and ITS4 ( White & al. Sequence data were generated for three nuclear and two mitochondrial loci. Additionally, herbarium material deposited in K and SAR was studied (herbarium codes according to Thiers 2019+).ĭNA was extracted by the CTAB method (Doyle & Doyle 1987) from a silica gel-dried piece of tissue from one individual per population. In both cases plants were documented by photographs.
SARAWAK BORNEO FULL
Also, herbarium vouchers were not collected if the only plants available were not in full bloom. In these cases only DNA samples were taken because for DNA analysis substantially less material is needed than for useful herbarium specimens. Because Thismia populations are sometimes composed of a few or even a single reproductive individual, voucher specimens were not always collected to prevent unnecessary harm to the population. Herbarium vouchers for this study are deposited in SAR and OL. Collected specimens were thoroughly compared with original drawings and descriptions given in protologues of representatives of Thismia sect. Morphological characters were studied using a hand lens (30–60× magnification), stereo microscope and macro photography. This study is based on material collected during January and February 2019 in Limbang and Kuching Divisions of Sarawak. 2018).ĭuring our field trip to Sarawak (Malaysian Borneo) in January and February 2019 we found two new species of Thismia and describe them in this article, which brings the number of Bornean species to 24. For example, 14 species were described in 2018 alone ( Chantanaorrapint & Suddee 2018 Dančák & al. The number of described species has been increasing rapidly over the last few years, which is probably one of the highest rates among angiosperms ( Stevens 2001+). Two main centres of Thismia diversity in Southeast Asia are located on the Malay Peninsula and in Borneo, with 16 and 22 recognized species, respectively ( Jonker 1948 Chantanaorrapint 2018 Dančák & al. 2018b) and is distributed mostly in the tropical regions of Asia, Australia and South America and extending into subtropical and temperate regions of Japan, New Zealand, Australia and the USA ( Merckx & al. The genus comprises about 85 species ( Dančák & al. ( Thismiaceae) is a genus of monocotyledonous plants that typically inhabit the understorey of tropical and subtropical forests. Version of record first published online on 10 March 2020 ahead of inclusion in April 2020 issue.
![sarawak borneo sarawak borneo](https://borneoadventure.com/v3/wp-content/uploads/2011/12/sarawak-melvin-ulu-ai-iban-girls-03.jpg)
coronata (Thismiaceae), two new species from Sarawak, Borneo. The species is assigned a preliminary conservation status of CR.Ĭitation: Dančák M., Hroneš M. Sarcosiphon and has been found at only one site near the village of Long Tuyo in Lawas district of northern Sarawak. The species is assigned a preliminary conservation status of VU according to IUCN criteria.
![sarawak borneo sarawak borneo](https://media.pangeatravel.nl/groot/groot/landschap-bako-national-park-Sarawak-Maleisie.jpg)
![sarawak borneo sarawak borneo](http://www.uky.edu/OtherOrgs/KPS/pages/conferences/asia2012/images/IMG_1563c.jpg)
aseroe until now, although the two species differ considerably. This species has been misidentified as T. Odoardoa and occurs at several localities in western Sarawak around the city of Kuching. coronata ( Thismiaceae), two new species from Sarawak (Malaysian Borneo) are described and illustrated.